[Frontiers in Bioscience, Landmark, 25, 526-535, Jan 1, 2020]

Forsythiaside A alleviates renal damage in adriamycin-induced nephropathy

Chan Lu1, She Feng Zheng1, Jinnv Liu1

1Department of Nephrology, Rui’an people’s Hospital and The Third Affiliated Hospital of Wenzhou Medical University. Rui’an, Zhejiang, 325200, P. R. China


1. Abstract
2. Introduction
3. Materials and methods
    3.1. Reagents
    3.2. Adriamycin induced nephropathy model
    3.3. Renal index analysis
    3.4. H&E staining
    3.5. TUNEL staining
    3.6. Western Blot analysis
    3.7. Oxidative stress analysis
    3.8. ELISA analysis
    3.9. Statistical analysis
4. Results
    4.1. FA ameliorates renal dysfunction in Adr-induced nephropathy
    4.2. FA reduces pathological damage and apoptosis in Adr-induced nephropathy
    4.3. FA alleviates Adr-induced oxidative stress
    4.4. FA inhibits inflammatory response in Adr-induced nephropathy and increases survival
    4.5. FA inhibits the activation of NF-kappaBp65/MIP-2 pathway in Adr-induced nephropathy
5. Discussion
6. References


Nephrotic syndrome (NS) is a common urinary system disease that carries a poor progrnosis due to nonresponsivess to current treatments. The purpose of this study was to investigate the therapeutic effects of Forsythiaside (FA) in a rat model of adriamycin-induced nephropathy (AN). Compared with control group, FA treatment significantly reduced proteinuria, serum creatinine and urea nitrogen levels and reduced the number of apoptotic cells in the kidneys. FA alleviated Adr induced renal injury, and increased the sactivity of superoxide dismutase (SOD), while decreasing malondialdehyde (MDA) and lactate dehydrogenase (LDH) in the serum. In addition, FA, in a dose-dependent manner, decreased the levels of NF-kappaBp65/MIP-2 in the kidenys, reduced the serum level of pro-inflammatory cytokines (IL-6, IL-1 β and TNF-alpha), and increased the survival rate of Adr treated rats. Taken together, the results show that FA alleviates renal dysfunction in adriamycin-induced nephropathy rats.


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Abbeviations: Adriamycin (Adr), Adriamycin nephropath (AN), Forsythoside A (FA), Nephrotic Syndrome (NS)

Key Words: Forsythiaside A, Adriamycin, Nephropathy, p65 NF-kappaB, MIP-2

Send correspondence to: Jinnv Liu, Department of Nephrology, Rui’an people’s Hospital and The Third Affiliated Hospital of Wenzhou Medical University. Room 4-3-202, Jiyun mountain road, Anyang street, Ruian, Zhejiang, 325200, P. R. China, Tel: 8613906646000, Fax: 057765866573, E-mail: jn3djt1@sina.com