[Frontiers in Bioscience 3, a11-15, February 1, 1998]

	[Frontiers in Bioscience 3, a11-15, February 1, 1998] Reprints PubMed CAVEAT LECTOR
	TARGETED INHIBITION OF HEPATITIS B VIRUS GENE EXPRESSION: A GENE THERAPY APPROACH Frank Y.-T. Tung and Steven W. Bowen Department of Infectious Disease and Microbiology, University of Pittsburgh, Pittsburgh PA 15261 Received 1/21/98 Accepted 1/28/98 TABLE OF CONTENTS 1. Abstract 2. Introduction 3. Materials and methods 3.1. Construction of retroviral vectors and producer lines 3.2. Transient assay of antisense-mediated inhibition of HBsAg 3.3. Northern blot analysis of antisense and sense RNA expressed in transduced HepG2 cells 4. Results 4.1 construction of retroviral vectors and producer lines encoding an antisense and sense copy of surface antigen. 4.2 inhibition of HBAg production by co-transfection assay 4.3 inhibition of HBsAg production in the antisense-transduced HepG2 cells 5. Discussion 6. Acknowledgment 7. References 8. Entire manuscript Key words: Hepatitis B Virus, Surface antigen, Gene therapy, Chronic hepatitis, Retroviral vector. Search OMIM (Online Mendelian Inheritance in Man) to find related articles This form requires a WWW Client such as Netscape, XMosaic, Lynx that supports fill-in forms. Enter one or more search keywords: To Search all fields, leave the following boxes unchecked. To narrow your search to certain specific fields, check the boxes next to those fields' names. SeeQuery Help for other ways to search. Title: OMIM Number: Allelic Variants: Text: References: Clinical Synopsis: Gene Map Disorder: Contributors: See only records which have been changed in the past Display at most

[Frontiers in Bioscience 3, a11-15, February 1, 1998]
Reprints
PubMed
CAVEAT LECTOR

TARGETED INHIBITION OF HEPATITIS B VIRUS GENE EXPRESSION: A GENE THERAPY APPROACH

Frank Y.-T. Tung and Steven W. Bowen

Department of Infectious Disease and Microbiology, University of Pittsburgh, Pittsburgh PA 15261

Received 1/21/98 Accepted 1/28/98

TABLE OF CONTENTS

1. Abstract
2. Introduction
3. Materials and methods: 3.1. Construction of retroviral vectors and producer lines
3.2. Transient assay of antisense-mediated inhibition of HBsAg
3.3. Northern blot analysis of antisense and sense RNA expressed in transduced HepG2 cells
4. Results: 4.1 construction of retroviral vectors and producer lines encoding an antisense and sense copy of surface antigen.
4.2 inhibition of HBAg production by co-transfection assay
4.3 inhibition of HBsAg production in the antisense-transduced HepG2 cells
5. Discussion
6. Acknowledgment
7. References
8. Entire manuscript

Key words: Hepatitis B Virus, Surface antigen, Gene therapy, Chronic hepatitis, Retroviral vector.

Search OMIM (Online Mendelian Inheritance in Man) to find related articles
This form requires a WWW Client such as Netscape, XMosaic, Lynx that supports fill-in forms.