Genes that induce replicative senescence

Entrez ID

Symbol

Method Used To
Cause Senescence

Cell type

Cell line

Cancer Line

22848

AAK1

Overexpression

Embryonic kidney, Lung fibroblast

HEK293T, IMR-90, MRC-5

No

51225

ABI3

Overexpression

Colon cancer, Thyroid cancer

ARO, WRO

Yes

203054

ADCK5

Overexpression

Embryonic kidney, Lung fibroblast

HEK293T, IMR-90, MRC-5

No

183

AGT

Knockout, Overexpression

Vascular smooth muscle

Primary cell

No

247

ALOX15B

Overexpression

Prostate epithelial

Primary cell

No

367

AR

Knockdown, Overexpression

Hair follicular keratinocyte

Primary cell

No

10095

ARPC1B

Overexpression

Osteosarcoma

SaOs-2

Yes

558

AXL

Overexpression

Embryonic kidney, Lung fibroblast

HEK293T, IMR-90, MRC-5

No

640

BLK

Overexpression

Embryonic kidney, Lung fibroblast

HEK293T, IMR-90, MRC-5

No

29117

BRD7

Knockdown

Foreskin fibroblast

BJ

No

857

CAV1

Knockdown

Fibroblast

Primary cell

No

284119

CAVIN1

Knockdown, Overexpression

Cervical cancer, Lung fibroblast

HeLa, IMR-90, WI-38

No

595

CCND1

Overexpression

Fibroblast

Hs68, WI-38

No

5129

CDK18

Overexpression

Embryonic kidney, Lung fibroblast

HEK293T, IMR-90, MRC-5

No

1026

CDKN1A

Knockout

Fibroblast

LF1

No

1027

CDKN1B

Overexpression

Lung fibroblast

TIG-3

No

1028

CDKN1C

Overexpression

Liver cancer

HepG2, SNU-398

Yes

1029

CDKN2A

Overexpression

Lung fibroblast

TIG-3

No

55602

CDKN2AIP

Overexpression

Lung fibroblast

MRC-5, TIG-1

No

1030

CDKN2B

Overexpression

Glioblastoma

T98G, U-251MG, U-373MG

Yes

1152

CKB

Overexpression

Embryonic kidney, Lung fibroblast

HEK293T, IMR-90, MRC-5

No

64506

CPEB1

Knockout, Overexpression

Foreskin fibroblast, Lung fibroblast

Primary cell, WI-38

No

3576

CXCL8

Overexpression

Foreskin fibroblast, Lung fibroblast

BJ, IMR-90, WI-38

No

3491

CYR61

Overexpression

Foreskin fibroblast, Lung fibroblast

BJ, IMR-90

No

1869

E2F1

Overexpression

Lung fibroblast

WI-38

No

26298

EHF

Overexpression

Lung fibroblast

WI-38

No

54206

ERRFI1

Overexpression

Lung fibroblast

WI-38

No

2130

EWSR1

Knockout

Hematopoietic stem

Primary cell

No

10922

FASTK

Overexpression

Embryonic kidney, Lung fibroblast

HEK293T, IMR-90, MRC-5

No

79791

FBXO31

Overexpression

Breast cancer

MCF-7

Yes

56287

GKN1

Overexpression

Gastric cancer

AGS, MKN1

Yes

2791

GNG11

Knockout, Overexpression

Lung fibroblast

TIG-7

No

2870

GRK6

Overexpression

Embryonic kidney, Lung fibroblast

HEK293T, IMR-90, MRC-5

No

3065

HDAC1

Overexpression

Cervical cancer

HeLa

Yes

220296

HEPACAM

Overexpression

Breast cancer

MCF-7

Yes

3101

HK3

Overexpression

Embryonic kidney, Lung fibroblast

HEK293T, IMR-90, MRC-5

No

3458

IFNG

Overexpression

Umbilical vein endothelial

Primary cell

No

3484

IGFBP1

Overexpression

Breast cancer

MCF-7

Yes

3488

IGFBP5

Knockout, Overexpression

Umbilical vein endothelial

Primary cell

No

3552

IL1A

Knockout

Umbilical vein endothelial

Primary cell

No

3621

ING1

Overexpression

Foreskin fibroblast

Hs68

No

3622

ING2

Knockout, Overexpression

Lung fibroblast

MRC-5

No

3661

IRF3

Overexpression

Foreskin fibroblast

BJ

No

3663

IRF5

Overexpression

Li Fraumeni syndrome fibroblast

MDAH041

Yes

3665

IRF7

Overexpression

Li Fraumeni syndrome fibroblast

MDAH041

Yes

3691

ITGB4

Knockdown

Umbilical vein endothelial

Primary cell

No

3705

ITPK1

Overexpression

Embryonic kidney, Lung fibroblast

HEK293T, IMR-90, MRC-5

No

3707

ITPKB

Overexpression

Embryonic kidney, Lung fibroblast

HEK293T, IMR-90, MRC-5

No

3984

LIMK1

Overexpression

Embryonic kidney, Lung fibroblast

HEK293T, IMR-90, MRC-5

No

5604

MAP2K1

Overexpression

Intestinal epithelial

HIEC-6

No

5608

MAP2K6

Overexpression

Osteosarcoma

U2OS

Yes

5609

MAP2K7

Overexpression

Embryonic kidney, Lung fibroblast

HEK293T, IMR-90, MRC-5

No

9064

MAP3K6

Overexpression

Embryonic kidney, Lung fibroblast

HEK293T, IMR-90, MRC-5

No

6885

MAP3K7

Overexpression

Embryonic kidney, Lung fibroblast

HEK293T, IMR-90, MRC-5

No

6300

MAPK12

Overexpression

Embryonic kidney, Lung fibroblast

HEK293T, IMR-90, MRC-5

No

22983

MAST1

Overexpression

Embryonic kidney, Lung fibroblast

HEK293T, IMR-90, MRC-5

No

4145

MATK

Overexpression

Embryonic kidney, Lung fibroblast

HEK293T, IMR-90, MRC-5

No

10445

MCRS1

Overexpression

Foreskin fibroblast, Lung fibroblast, Mammary epithelial

H184B5F5/M10, Hs68, IMR-90

No

126308

MOB3A

Overexpression

Embryonic kidney, Lung fibroblast

HEK293T, IMR-90, MRC-5

No

23515

MORC3

Overexpression

Lung fibroblast

WI-38

No

10934

MORF4

Overexpression

Fibrosarcoma

A9, HT1080

Yes

4598

MVK

Overexpression

Embryonic kidney, Lung fibroblast

HEK293T, IMR-90, MRC-5

No

10608

MXD4

Overexpression

Lung fibroblast

WI-38

No

65220

NADK

Overexpression

Embryonic kidney, Lung fibroblast

HEK293T, IMR-90, MRC-5

No

10397

NDRG1

Knockdown

Liver cancer

Hep3B, HepG2, Huh7, PLC/PRF/5

Yes

4814

NINJ1

Overexpression

Hepatoma

Huh7

Yes

50507

NOX4

Knockdown, Overexpression

Breast epithelial, Lung fibroblast

MCF-12A, TIG-3

No

9891

NUAK1

Knockout, Overexpression

Lung fibroblast

WI-38

No

5015

OTX2

Overexpression

Medulloblastoma

D283, D341, D425, D458, D556, DAOY, MED8A, UW228-2

Yes

10298

PAK4

Overexpression

Embryonic kidney, Lung fibroblast

HEK293T, IMR-90, MRC-5

No

55193

PBRM1

Knockdown

Foreskin fibroblast

BJ

No

7703

PCGF2

Knockout, Overexpression

Foreskin fibroblast, Lung fibroblast

BJ, MRC-5, WI-38

No

149420

PDIK1L

Overexpression

Embryonic kidney, Lung fibroblast

HEK293T, IMR-90, MRC-5

No

5170

PDPK1

Overexpression

Embryonic kidney, Lung fibroblast

HEK293T, IMR-90, MRC-5

No

23037

PDZD2

Overexpression

Breast cancer, Liver cancer, Prostate cancer

DU145

Yes

51588

PIAS4

Overexpression

Lung fibroblast

WI-38

No

23533

PIK3R5

Overexpression

Embryonic kidney, Lung fibroblast

HEK293T, IMR-90, MRC-5

No

5315

PKM

Overexpression

Embryonic kidney, Lung fibroblast

HEK293T, IMR-90, MRC-5

No

22925

PLA2R1

Knockout, Overexpression

Lung fibroblast

IMR-90, WI-38

No

10654

PMVK

Overexpression

Embryonic kidney, Lung fibroblast

HEK293T, IMR-90, MRC-5

No

87178

PNPT1

Overexpression

Melanoma

FO-1, HO-1, MeWo

Yes

5460

POU5F1

Overexpression

Mesenchymal stromal

Primary cell

No

5580

PRKCD

Knockdown, Overexpression

Lung fibroblast

TIG-1

No

5629

PROX1

Overexpression

Liver cancer

Hep3B, HepG2, Mahlavu, SK-HEP1

Yes

9232

PTTG1

Overexpression

Foreskin fibroblast, Lung fibroblast

BJ, IMR-90, WI-38

No

6041

RNASEL

Knockout, Overexpression

Lung fibroblast

WI-38

No

27330

RPS6KA6

Knockdown, Overexpression

Colon cancer, Lung fibroblast

IMR-90

No

861

RUNX1

Overexpression

Foreskin fibroblast

Hs68

No

5054

SERPINE1

Knockdown

Foreskin fibroblast

BJ, Primary cell

No

150094

SIK1

Overexpression

Embryonic kidney, Lung fibroblast

HEK293T, IMR-90, MRC-5

No

23309

SIN3B

Knockout, Overexpression

Lung fibroblast

IMR-90

No

64849

SLC13A3

Overexpression

Lung fibroblast, Renal tubular

MRC-5, WI-38

No

6598

SMARCB1

Overexpression

Malignant rhabdoid tumor

G-401

Yes

64750

SMURF2

Overexpression

Foreskin fibroblast, Lung fibroblast, Skin fibroblast

BJ, WI-38, WS1

No

8470

SORBS2

Overexpression

Fibroblast, Keratinocyte

Primary cell

No

6657

SOX2

Overexpression

Colon cancer, Embryonic kidney

HCT116, HEK293T, WiDr

Yes

6667

SP1

Overexpression

Lung fibroblast

2BS

No

10927

SPIN1

Overexpression

Cervical cancer

HeLa

Yes

8405

SPOP

Knockdown, Overexpression

Lung fibroblast

IMR-90

No

6720

SREBF1

Overexpression

Fibroblast

Primary cell

No

6777

STAT5B

Overexpression

Lung fibroblast

IMR-90

No

282974

STK32C

Overexpression

Embryonic kidney, Lung fibroblast

HEK293T, IMR-90, MRC-5

No

83931

STK40

Overexpression

Embryonic kidney, Lung fibroblast

HEK293T, IMR-90, MRC-5

No

6850

SYK

Overexpression

Melanoma

501-mel, A375, SK-MEL-28

Yes

7023

TFAP4

Overexpression

Breast cancer, Retinal pigment epithelial

hTERT-RPE, MCF-7

Yes

7041

TGFB1I1

Knockout, Overexpression

Fibroblast

KMST-6, SUSM-1

No

7098

TLR3

Overexpression

Fibroblast, Keratinocyte

Primary cell

No

9966

TNFSF15

Knockout, Overexpression

Endothelial progenitor

Primary cell

No

7150

TOP1

Knockout

Lung fibroblast

IMR-90, WI-38

No

8626

TP63

Knockout

Foreskin fibroblast

BJ

No

7297

TYK2

Overexpression

Embryonic kidney, Lung fibroblast

HEK293T, IMR-90, MRC-5

No

27287

VENTX

Knockdown, Overexpression

Cervical cancer, Embryonic kidney, Lung fibroblast, Lymphoblastic leukemia, Osteosarcoma

HEK293T, HeLa, IMR-90, Nalm16, U2OS

Yes

51384

WNT16

Knockdown

Lung fibroblast

MRC-5

No

7538

ZFP36

Overexpression

Cervical cancer

HeLa

Yes

8553

BHLHE40

Overexpression

Breast cancer, Esophageal cancer

EC9706, MCF-7

Yes

2626

GATA4

Knockdown, Overexpression

Foreskin fibroblast, Lung fibroblast

BJ, IMR-90

No

1432

MAPK14

Knockout

Lung fibroblast

MRC-5, WI-38

No

6787

NEK4

Knockdown

Foreskin fibroblast

BJ

No

7157

TP53

Overexpression

Bladder cancer

EJ

Yes

80019

UBTD1

Overexpression

Breast cancer, Colorectal cancer, Gastric cancer, Gastric epithelial, Lung fibroblast, Prostate cancer

AGS, GES-1, KATO-III, LoVo, MCF-7, MKN28, MKN45, MRC-5, NCI-N87, PC-3, SGC-7901, SNU-1, SNU-16

Yes

Reference: HAGR