[Frontiers in Bioscience E5, 551-557, January 1, 2013]

The biological evaluation of ADMA/SDMA and eNOS in patients with ACHF

Lorenza Speranza1, Mirko Pesce1, Sara Franceschelli1, Tonino Bucciarelli2, Sabina Gallina3, Graziano Riccioni4,Antonia Patruno1 and Mario Felaco1

1Department of Medicine and Science of aging, University G. D'Annunzio, Chieti Italy, 2Department of Biomedical Science, Clinical Biochemistry, G. D'Annunzio University Chieti, Italy, 3Cardiology Department SS Annunziata Hospital, Via dei Vestini-Chieti Italy, 4San Camillo de Lellis' Hospital, Cardiology Unit, Italy


1. Abstract
2. Introduction
3. Materials and methods
3.1 .Study design
3.2. Patient population
3.3. Sample collection, storage and preparation
3.4. Isolation of human peripheral adherent mononuclear cells
3.5. Western blot analysis for eNOS
3.6. eNOS activity
3.7. Determination of O2-
3.8. Biochemical analysis
3.9. Echocardiographic-doppler evaluation
4. Statistical analysis
5. Results
5.1. Baseline characteristic of study population
5.2. HPLC results
5.3. eNOS expression and activity
5.4. O2- production
6. Discussion
7. Acknowledgment
8. References


The aim of this study was to investigate the effects of acute pharmacological treatment on the plasma levels of l-arginine, asymmetrical dimethylarginine (ADMA), and symmetrical dimethylarginine (SDMA). We also investigated the related effects on endothelial nitric oxide synthase (eNOS) expression and activity and cytochrome c oxidase activity in the primary blood mononuclear cells (PBMCs) isolated from patients with acute congestive heart failure (ACHF). Compared to pre-treatment values, ADMA, SDMA, and l-arginine plasma levels were significantly higher after pharmacological treatment (ADMA, 0.82 versus 0.43 ÁM; SDMA, 1.52 versus 1.12 ÁM; l-arginine, 1.78 versus 1.29 ÁM; p < 0.01. In addition, the levels of eNOS expression and activity were decreased after pharmacological treatment, while cytochrome c oxidase activity resulted in higher O2- production. In the PBMCs isolated from patients with acute congestive heart failure (ACHF) and impaired renal function, higher SDMA and ADMA levels were more evident after therapy, as were reduced expression and activity of eNOS. Increased O2- produced after treatment may be involved in impaired recovery of cardiac function associated with higher plasma levels of SDMA.