[Frontiers in Bioscience E3, 871-878, June 1, 2011]

Reference values of blood cell counts in the first days of life

Giovanni Melioli1, Francesco Maria Risso2,5, Andrea Sannia2,5, Giovanni Serra2,5, Roberto Bologna1, Michele Mussap3,5, Salvatore Mangraviti1, Patrizia Fortini1, Fabio Facco1, Giorgio Reggiardo4, Giuseppe Buonocore5,7, Giovanni Corsello5,8, Vassilios Fanos5,6, Antonello Del Vecchio9, Claudio Fabris10, Diego Gazzolo5,11

1Clinical Pathology Laboratories, G. Gaslini Children's Hospital, Genoa, Italy, 2Department of Maternal, Fetal and Neonatal Medicine G. Gaslini Children's Hospital, Genoa, Italy, 3Laboratory of Clinical Biochemical Medicine, S. Martino, Genoa, Italy, 4Biometric Unit, Medi-Service srl, Genoa, Italy, 5Neonatal Clinical and Biochemical Italian Research Group from Italian Society of Clinical Biochemistry (SiBioC) and Italian Society of Neonatology (SIN), 6Neonatal Intensive Care Unit University of Cagliari, Cagliari, Italy, 7Department of Maternal, Fetal, Neonatal and Reproductive Medicine University of Siena, Siena, Italy, 8Department of Maternal, Fetal and Neonatal Medicine University of Palermo, Palermo, Italy, 9Neonatal Intensive Care Unit Di Venere Hospital, Bari, Italy, 10Chair of Neonatog, University of Turin, Turin, Italy, 11Neonatal Intensive Care Unit C. Arrigo Children's Hospital, Alessandria, Italy


1. Abstract
2. Introduction
3. Materials and methods
3.1. Evaluation of sample size
3.2. Blood Cells Collection
3.3. Laboratory Procedure
3.4.Quality Controls
3.5. Statistical Analysis
4. Results
4.1. Quality Controls
4.2. Study Populations
4.3. Hematological Parameters
5. Discussion
6. References


The lack of updated neonatal reference values for hematological parameters impacts significantly with clinical management of both healthy and sick newborns. The present pilot study was thus aimed at assessing updated hematological Italian reference values in late preterm and term newborns. From January 2004 to December 2008 hematological laboratory tests were performed in 1175 newborns (820 healthy and 355 sick controls) between 33-41 weeks of gestation, during the first four days after birth. Hematological parameters were sorted for gender and gestational age and statistically analyzed. No gender-related differences were observed at different weeks of gestation and no significant differences were found when study population was sub-grouped for late preterm and term newborns. During the first 4 days of life erythrocytes and platelets remained stable whilst white blood cell counts and differentials were significantly modified. This study shares updated reference values for hematological parameters in the early phases after birth and offers additional support for improving the management of sick infants.