[Frontiers in Bioscience E3, 33-45, January 1, 2011]

Single nucleotide polymorphisms in IL-4Ra,IL-13 and STAT6 genes occurs in brain glioma

Zhenchao Ruan1, Yao Zhao3, Lili Yan1, Hongyan Chen3, Weiwei Fan3, Juxiang Chen4, Qihan Wu5, Ji Qian3, Tianbao Zhang6, KeKe Zhou2, Yin Mao2, Liangfu Zhou2, Yan Huang1, Daru Lu3

1State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai, 200433, People's Republic of China, 2Department of Neurosurgery, Huashan Hospital, Fudan University, Shanghai, 201206, People 's Republic of China, 3State Key Laboratory of Genetic Engineering and Key Laboratory of Contemporary Anthropology, School of Life Sciences, Fudan University, Shanghai, 200433, People 's Republic of China, 4Department of Neurosurgery, ChangZheng Hospital, Second Military Medical University, Shanghai Neurosurgical Institute, Shanghai 200003, People's Republic of China, 5School of Life Science, East China Normal University, Shanghai 200062, People 's Republic of China, 6Department of Toxicology, Shanghai Second Military Medical University, Shanghai, 200433, People 's Republic of China

TABLE OF CONTENTS

1.Abstract
2.Introduction
3.Methods and materials
3.1. Study population
3.2.Questionnaire
3.3.Laboratory genotyping
3.4. Statistical analysis
4.Results
4.1.Characteristics of study populations
4.2.Association study without stratification
4.3.Association analysis after stratification
4.4.Haplotype analysis for STAT6
4.5. Gene-Gene interaction between IL-13 and IL-4Ra for susceptibility to glioma
5.Discussion
6.Acknowledgements
7.References

1.ABSTRACT

Gliomas are aggressive brain tumor. Association studies were consistent for an inverse association between asthma and allergic conditions (IgE levels) and risk of glioma. Studies reported that the IL-4Ra, IL-13 and STAT6 genes played a relatively strong role in IgE production or allergy. This population-based case-control study aimed to find potential association between single nucleotide polymorphisms IL-13rs20541, IL-4Rars1801275 and glioma susceptibility in population, as well as STAT6 rs1059513 and STAT6 rs324015. Among non-smokers, homozygote GG of STAT6 4610A/G showed an increased association with risk of glioma compared with AA (adjusted OR=1.691, 95%CI=1.152-2.481, p=0.007, corrected p=0.028), and the haplotype with A allele at rs1059513 and G allele at rs324015 was revealed to increase glioma risk significantly (OR=1.321,95%CI= 1.081-1.614, p=0.007,corrected p=0.028). GG genotype of STAT6 4610A/G was a significant risk factor compared with AA in glioblastoma (adjusted OR=1.856, 95%CI=1.153-2.987, p=0.011, corrected p=0.044). GG of STAT6 4610A/G was significantly related to increased WHO IV risk compared with AA (adjusted OR=1.591,95%CI=1.030-2.459, p=0.036, corrected p=0.144). Interaction between IL-13 Arg130Gln and IL-4Ra Gln576Arg was observed in decreasing glioma risk (p=0.045).