Tanimoto K, Sugiyama F, Goto Y, Ishida J, Takimoto E, Yagami K, Fukamizu A, Murakami K:
Angiotensinogen-deficient mice with hypotension.
Journal of Biological Chemistry 1994 Dec 16;269(50):31334-7
ABSTRACT
The renin-angiotensin system is an enzymatic cascade that produces a
potent vasoconstrictor octapeptide angiotensin II, through its
physiologically inactive intermediate decapeptide angiotensin I,
from their precursor angiotensinogen. In the present study, we
generated angiotensinogen-deficient mice by homologous recombination
in mouse embryonic stem cells. These mice do not produce
angiotensinogen in the liver, resulting in the complete loss of
plasma immunoreactive angiotensin I. The systolic blood pressure of
the homozygous mutant mice was 66.9 +/- 4.1 mm Hg, significantly
lower than that of wild-type mice (100.4 +/- 4.4 mm Hg). This
profound hypotension in angiotensinogen-deficient mice demonstrates
an indispensable role for the renin-angiotensin system in
maintaining blood pressure.
