Defective T cell receptor signaling in mice lacking the thymic isoform of p59fyn.

Appleby MW, Gross JA, Cooke MP, Levin SD, Qian X, Perlmutter RM:
Defective T cell receptor signaling in mice lacking the thymic isoform of p59fyn.
Cell 1992 Sep 4;70(5):751-63

ABSTRACT
Considerable evidence supports the hypothesis that the nonreceptor protein tyrosine kinase p59fyn participates in signal transduction from the T cell receptor (TCR). To examine this hypothesis in detail, we have produced mice that lack the thymic isoform of p59fyn but retain expression of the brain isoform of the protein. fynTnull mice exhibit a remarkably specific lymphoid defect: thymocytes are refractile to stimulation through the TCR with mitogen or antigen, while peripheral T cells, following what appears to be a normal maturation sequence, reacquire significant signaling capabilities. These data confirm that p59fynT plays a pivotal role in TCR signal transduction and demonstrate that additional developmentally regulated signaling components also contribute to TCR-induced lymphocyte activation.