P selectin
Defects in leukocyte behaviour
Elevated number of circulating neutrophils- Total absence of leukocyte rolling in mesenteric venules
- Delayed recruitment of neutrophils
Normal fertility
Postcapillary venule rolling in homozygotes significantly lower in basal and post-ischemia-reperfusion Deletion of P-selectin gene did not eliminate rolling Similar shear rates and leukocyte-endothelial adhesion
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Yamada, 1995
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Rolling in P-selectin-deficient mice is reduced but not eliminated in the dorsal skin.
Blood 86, 3487-3492 (1995) [96027517]
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Exhibition of peripheral neutrophilia Delayed clearance of (51Chromium-labeled) neutrophils No alteration in lymphocyte differentiation, distribution, or adhesion Fewer rolling cells and reduced velocity Threefold increase of peritoneal macrophages in wild-type in peritonitis model Baseline levels of peritoneal macrophages in homozygotes in peritonitis model
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Johnson, 1995
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Blood cell dynamics in P-selectin-deficient mice.
Blood 86, 1106-1114 (1995) [95345461]
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P- and E-selectin deficient mice
Extreme leukocytosis
Elevated cytokine levels
Increased bone marrow and spleen granulocytopoiesis
Erythropoiesis partially translocated to spleen
Virtual lack of leukocyte rolling
Low extravasation at sites of inflammation
High susceptability to opportunistic bacterial infection
| Line Name | DNA Construct | |
|---|---|---|
| 1. | P-selectin (+/-) | An amplified mouse genomic library constructed from the liver DNA of black agouti 129Sv mouse strain (kindly provided by Drs. H. Wu and R. Jaenisch (G00-100-762) was scr.... |
| 2. | P-selectin (-/-) | An amplified mouse genomic library constructed from the liver DNA of black agouti 129Sv mouse strain (kindly provided by Drs. H. Wu and R. Jaenisch (G00-100-762) was scr.... |
| 3. | Sele (+/-) | Murine E-selectin genomic clones were isolated from lambda libraries derived from either 129SV DNA (Stratagene, Incorporated) or BALB/c DNA (Clonetec, Incorporated). In.... |
| 4. | Sele (-/-) | Murine E-selectin genomic clones were isolated from lambda libraries derived from either 129SV DNA (Stratagene, Incorporated) or BALB/c DNA (Clonetec, Incorporated). In.... |
