Int-1
Heterozygotes are normal and fertile
Homozygotes exhibit phenotypes ranging from death, either before birth (Thomas and Capecchi, 1990) or shortly thereafter (McMahon and Bradley, 1990), to survival with severe ataxia (Thomas and Capecchi, 1990)
Developmental abnormalities of the brain in homozygotes
Severe abnormalities in development of mesencephalon and metencephalon (Thomas and Capecchi, 1990) Absence of most of midbrain and some rostral metencephalon in day 9.5 fetuses (McMahon and Bradley, 1990) Virtually no midbrain and no cerebellum in late gestation fetuses (McMahon and Bradley, 1990) Death within 24 hours of birth (McMahon and Bradley, 1990)
McMahon and Bradley, 1990
Thomas and Capecchi, 1990
| Line Name | DNA Construct | |
|---|---|---|
| 1. | int-1(neo) (+/-) | The targeting vector used to mutate int-1, pINT-1-N/TK contains 13.5 kb of mouse genomic DNA surrounding the int-1 structural gene, which is interrupted at the |
| 2. | int-1(neo) (-/-) | The targeting vector used to mutate int-1, pINT-1-N/TK contains 13.5 kb of mouse genomic DNA surrounding the int-1 structural gene, which is interrupted at the |
| 3. | Wnt-1 (int-1) (+/-) | The neo cassette used was a modification of pMC1neopA (G00-354-270). Initially, a neo-tk cassette was constructed by cloning a |
| 4. | Wnt-1 (int-1) (-/-) | The neo cassette used was a modification of pMC1neopA (G00-354-270). Initially, a neo-tk cassette was constructed by cloning a |
