Inducible nitric oxide synthase (iNOS)

Inducible nitric oxide synthase (iNOS)

Viable
Fertile
No evident histopathological abnormalities
Susceptibility to Leishmania major infection
Significantly stronger Th1 type of immune response
Reduced nonspecific inflammatory response to carrageenin
Resistance to lipopolysaccharide-induced mortality

Wei et al, 1995

Homozygous mice

Failure to restrain replication of Listeria monocytogenes in vivo or lymphoma cells in vitro

Marked attenuation in the fall of central arterial blood pressure due to LPS

LPS-induced liver damage in unanesthesized mice

Shock and death in anesthesized wild-type mice due to bacterial endotoxic lipopolysaccharide

Normal death rate of mice when primed with Propionobacterium acnes and challenged with LPS

MacMicking, 1995: Altered responses to bacterial infection and endotoxic shock in mice lacking inducible nitric oxide synthase.
Cell 8145, 641-650 (1995) [95277851]
(View Citation format,Abstract format,MEDLARS format,ASN.1 format,Save As..., or 30 Related MEDLINE citations)

Indistinguishable appearance and histology between homozygous mice and wild-type mice

Lack of nitric oxide production upon lipopolysaccharide and IFN gamma treatment in homozygotes

Absence of enzyme activity in cell lysates

No increase in nitrite plus nitrate levels in homozygotes treated with lethal lipopolysaccharide dosage

No survival advantage of homozygotes over wild-type

Laubach, 1995: Mice lacking inducible nitric oxide synthase are not resistant to lipopolysaccharide-induced death.
Proc Natl Acad Sci U S A 92, 10688-10692 (1995) [96068681]
(View Citation format,Abstract format,MEDLARS format,ASN.1 format,Save As..., or 30 Related MEDLINE citations)

METHODOLOGY ( FROM T-BASE)

((inos))

Line Name DNA Construct

1. iNOS (+/-) A replacement type targeting construct was prepared from 129/Sv genomic DNA. A single targeted clone was identified among 636 screened after two independent electropora....
2. iNOS A replacement type targeting construct was prepared from 129/Sv genomic DNA. A single targeted clone was identified among 636 screened after two independent electropora....

Database links