Hdh ex 5
Homozygous mice
Initiation of gastrulation
Absence of somite formation and organogenesis
Death before embryonic day 8.5 Heterozygous mice
Viable
Increased motor activity and cognitive deficits
Significant neuronal loss in subthalamic nucleus
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Nasir, 1995
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Targeted disruption of the Huntington's disease gene results in embryonic lethality and behavioral and morphological changes in heterozygotes.
Cell 81, 811-823 (1995) [95292348]
(View Citation format,Abstract format,MEDLARS format,ASN.1 format,Save As..., or 30 Related MEDLINE citations)
| Line Name | DNA Construct | |
|---|---|---|
| 1. | Hdh (ex5) (-/-) | A phage genomic library from the 129/Sv mouse strain (Stratagene) was screened using a probe encompassing exon 5 of the murine Hdh cDNA (Lin et al., 1994; G00-311-167)..... |
| 2. | Hdh (exon 4/5) (-/- | To disrupt Hdh in ES cells, vector pPNT-Hdh 4/5 was used which removes exons 4 and 5 and replaces them with the bacterial neo gene. The Hdh targeting vector {pPNT-Hdh 4.... |
| 3. | Hdh (prex1) (+/-) | To obtain a hybridization probe by RT-PCR, Zeitlin et al., 1995 (G00-681-762) used poly(A)+ RNA isolated from human kidney and HeLa cells and the following primers: Reve.... |
| 4. | Hdh (prex1) (-/-) | To obtain a hybridization probe by RT-PCR, Zeitlin et al., 1995 (G00-681-762) used poly(A)+ RNA isolated from human kidney and HeLa cells and the following primers: Reve.... |
| 5. | Hdh (exon4/5) (+/-) | To disrupt Hdh in ES cells, vector pPNT-Hdh 4/5 was used which removes exons 4 and 5 and replaces them with the bacterial neo gene. The Hdh targeting vector {pPNT-Hdh 4.... |
| 6. | Hdh (ex5) (+/-) | A phage genomic library from the 129/Sv mouse strain (Stratagene) was screened using a probe encompassing exon 5 of the murine Hdh cDNA (Lin et al., 1994; G00-311-167)..... |
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P42859
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HUNTINGTIN (HUNTINGTON DISEASE PROTEIN) (HD PROTEIN).
gi|1170193|sp|P42859|HD_MOUSE [1170193]
(View GenPept format,Report format,FASTA format,ASN.1 format,Graphical View,Save As..., 2 MEDLINE links, or 20 Related Protein sequences )
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MUSHDH cds1
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Huntington's Disease gene homologue
gi|454417 [454417]
(View GenPept format,Report format,FASTA format,ASN.1 format,Graphical View,Save As..., 1 MEDLINE link, 20 Related Protein sequences, or 1 nucleotide link )