Csk
Developmental arrest in the 10-12 somite stage
Growth retardation and necrosis in neural tissue
Enhanced p60c-src, p59fyn and p53/p56lyn
Necrosis of embryos at E10.5
Imamoto and Soriano, 1993
Nada et al., 1993
| Line Name | DNA Construct | |
|---|---|---|
| 1. | CSK (+/-) | |
| 2. | CSK (-/-) | |
| 3. | CSK/pCZ-DT (+/-) | To introduce mutations into the csk gene by homologous recombination in ES cells, 2 types of targeting vector were constructed. The pCZ-DT vector has lacZ and neo[r] (n.... |
| 4. | CSK/pNZ-DT (+/-) | In pNZ-DT, the DNA fragment from a Kpnl site in the 5'-non-coding exon to an Ncol site in the kinase domain was replaced with lacZ and neo(r) genes. Since the translatio.... |
| 5. | csk (+/-) | A neo expression cassette (PGKneobpA) was used to replace a 4-kb Kpnl-Xhol fragment that contains all c-Src kinase (csk) coding sequences except 10 amino acids at the C-.... |
| 6. | csk (-/-) | A neo expression cassette (PGKneobpA) was used to replace a 4-kb Kpnl-Xhol fragment that contains all c-Src kinase (csk) coding sequences except 10 amino acids at the C-.... |
| 7. | CSK/pCZ-DT (-/-) | To introduce mutations into the csk gene by homologous recombination in ES cells, 2 types of targeting vector were constructed. The pCZ-DT vector has lacZ and neo[r] (n.... |
| 8. | CSK/pNZ-DT (-/-) | In pNZ-DT, the DNA fragment from a Kpnl site in the 5'-non-coding exon to an Ncol site in the kinase domain was replaced with lacZ and neo(r) genes. Since the translatio.... |
