CD40

Normal phenotype and normal number of T and B cells
Normal response by B cells to lipopolysaccharide with IL-4
Normal response to T-cell independent antigens 2,4,6-trinitrophenyl-LPS and TNP-Ficoll
Lack of proliferation and isotype switching by B cells in vitro in response to soluble CD40 ligand with interleukin 4
Failure to mount an antigen-specific antibody response
Failure to develop germinal centers following immunization with the T-cell dependent antigen keyhole limpet hemocyanin
Absence of IgE and severe decrease of IgG1 and IgG2a in the serum of young (6-8weeks) mice

Castigli, 1994

CD40-deficient mice generated by recombination-activating gene-2-deficient blastocyst complementation.
Proc Natl Acad Sci U S A 91, 12135-12139 (1994) [95083656]
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( (cd40))

	Line Name	DNA Construct
1.	CD40 (-/-)	Castigli et al (1994) isolated from a 129 Sv genomic library a 13-kb genomic DNA and prepared a targeting construct that eliminated exons 7, 8 , and 9 (coding for the tr....
2.	vav (-/-)	An 8.1 kb BamHI fragment containing several exons of the vav gene homologous to the dbl proto-oncogene was isolated from a 129/Sv mouse genomic library. An e....
3.	vav (-/-)	Five independent vav genomic phage clones were obtained from a mouse strain 129 library in lambda FixII (Stratagene). Selected fragments were cloned into pUC18 construc....
4.	Hlx (-/-)	In the targeting vector, which has 7.5 kb of Hlx sequences in its left arm and 2.8 kb in its right, a neo(R) expression cassette containing a phosphoglycerol kinase-1 pr....