TRIS (HYDROXYMETHYL) AMINOMETHANE BUFFER
|
mL 0.1 N HCl
Added |
Resulting pH at 23 °C |
Resulting pH at 37 °C |
|
5.0 |
9.10 |
8.95 |
|
7.5 |
8.92 |
8.78 |
|
10.0 |
8.74 |
8.60 |
|
12.5 |
8.62 |
8.48 |
|
15.0 |
8.50 |
8.37 |
|
17.5 |
8.40 |
8.27 |
|
20.0 |
8.32 |
8.18 |
|
22.5 |
8.23 |
8.10 |
|
25.0 |
8.14 |
8.00 |
|
27.5 |
8.05 |
7.90 |
|
30.0 |
7.96 |
7.82 |
|
32.5 |
7.87 |
7.73 |
|
35.0 |
7.77 |
7.63 |
|
37.5 |
7.66 |
7.52 |
|
40.0 |
7.54 |
7.40 |
|
42.5 |
7.36 |
7.22 |
|
45.0 |
7.20 |
7.05 |