FRONTIERS IN BIOSCIENCE;
WHOLE BLOOD, SERUM AND PLASMA CHEMISTRY:A-E



Table of Conents

 

TYPICAL REFERENCE INTERVALS

COMPONENT

SYSTEM

CONVENTIONAL UNITS

FACTOR

RECOMMENDED SI UNITS

Acetoacetic Acid

Qualitative

Quantitative

 

Serum

Serum

 

Negative

0.2-1.0 mg/dL

 

---

97.95

 

Negative

20-100 mmol/L

Acetone

Qualitative

Quantitative

 

Serum

Serum

 

Negative

0.3-2.0 mg/dL

 

---

172.95

 

Negative

20-340 mmol/L

Albumin

Qualitative

 

Serum

 

3.2-4.5 g/dL(salt fractionation)

3.2-5.6 g/dL(electrophoresis)

3.8-5.0 g/dL (dye binding)

 

10

 

32-45 g/L

32-56 g/L

38-50 g/L

Alcohol, ethyl

Serum or Whole Blood

Negative--but presented as mg/dL

0.2171

Negative --but present as mmol/L

Aldolase

Serum

adults

children

newborn

 

3-8 Sibley-Lehninger U/dL at 37 oC

Approximately 2 times adult levels

Approximately 4 times adult levels

 

7.4

 

22-59 mU/L at 37oC

Approximately 2 times adult levels

Approximately 4 times adult levels

a -Amino acid nitrogen

Serum

3.6-7.0 U/dL

0.7139

2.6-5.0 mmol/L

d- Aminolevulinic acid

Serum

0.01-0.03mg/dL

76.26

0.8-2.3mmol/L

Ammonia

Plasma

20-120 mg/dL(diffusion)

40-80 mg/dL(enzymatic method)

12-48 mg/dL(resin method)

0.5872

12-70 m mol/L

23-47 mmol/L

7-28 mmol/L

Amylase

Serum

16-120 Somogyi units/dL

1.85

30-220 U/L

Argininosuccinate lyase

Serum

0-4 U/dL

10

0-40 U/L

Arsenic++

Whole blood

<7 mg/dL

0.05055

<0.4 mmol/L

Ascorbic Acid (vitamin C)

Plasma

Whole blood

0.6-1.6 mg/dL

0.7-2.0 mg/dL

56.78

34-91 mmol/L

40-114 mmol/L

Barbiturates

Serum, Plasma or Whole blood

Negative

---

Negative

Base excess

Whole blood

Male

Female

 

-3.3 to +1.2 mEq/L

-2.4 to +2.3 mEq/L

 

1

 

-3.3 to +1.2 mmol/L

-2.4 to +2.3 mmol/L

Base, total

Serum

145-160 mEq/L

1

145-160 mmol/L

Bicarbonate

Plasma

21-28 mmol/L

1

21-28 mmol/L

Bile acids

Serum

0.3-3.0 mg/dL

10

3.0-30.0 mg/L

Bilirubin

Direct (conjugated)

Indirect (unconjugated)

Total

Newborns Total

Serum

 

<0.3 mg/dL

0.1-1.0 mg/dL

0.1-1.2 mg/dL

1.0-12.0 mg/dL

 

17.10

 

<5 mmol/L

2-17 mmol/L

2-21 mmol/L

17-205 mmol/L

Blood Gases

PH

Pco2

Po2

 

Whole Blood

Whole Blood

Whole Blood

 

7.38-7.44 (arterial)

7.36-7.41(venous)

35-40mm Hg (arterial)

40-45mm Hg (venous)

95-100mm Hg(arterial)

 

1

0.1333

0.1333

 

7.38-7.44

7.36-7.41

4.7-5.3 kPa

5.3-6.0 kPa

12.7-13.3 kPa

Bromide

Serum

<5 mg/dL

0.125

<0.63 mmol/L

Calcium

Ionized

 

Total

 

Serum

 

Serum

 

4.0-4.8 mg/dL

2.0-2.4 mEq/L

30-58% of total

9.2-11.0 mg/dL

4.6-5.5 mEq/L

 

0.2500

0.5000

0.01

0.2500

0.5000

 

1.00-1.20 mmol/L

0.30-1.58 of total

2.30-2.74 mmol/L

Carbon Dioxide(CO2 content)

Whole Blood (arterial)

Plasma or Serum (arterial)

19-24 mmol/L

21-28 mmol/L

 

1

19-24 mmol/L

21-28 mmol/L

Carbon Dioxide

Whole blood (venous)

Plasma or Serum(venous)

22-26 mmol/L

24-30 mmol/L

1

22-26 mmol/L

24-30 mmol/L

CO2 combining power

Plasma or Serum(venous)

24-30 mmol/L

1

24-30 mmol/L

CO2 partial pressure (Pco2)

Whole Blood (arterial)

Whole Blood (venous)

35-40 mm Hg

40-45 mm Hg

0.1333

4.7-5.3 kPa

5.3-6.0 kPa

Carbonic acid (H2CO3)

Whole Blood (arterial)

Whole Blood (venous)

Plasma (venous)

1.05-1.45 mmol/L

1.15-1.50 mmol/L

1.02-1.38 mmol/L

1

1.05-1.45 mmol/L

1.15-1.50 mmol/L

1.02-1.38bmmol/L

Carboxyhemoglobin (carbon monoxide hemoglobin)

Whole blood

suburban nonsmokers

smokers

heavy smokers

 

<1.5% saturation of hemoglobin

1.5-5.0% saturation

5.0-9.0% saturation

 

0.01

 

Fraction hemoglobin saturated: <0.015

0.015-0.050

0.050-0.090

Carotene beta

Serum

40-200Fg/dL

0.01863

0.73.7 mmol/L

Ceruloplasmin

Serum

23-50 mg/dL

10

230-500 mg/L

Chloride

Serum

95-103 mEq/L

1

95-103mmol/L

Cholesteral

Total

Esters

 

Serum

Serum

 

150-250 mg/dL (varies with diet, sex, and age)

65-75% of total cholesterol

 

0.02586

0.01

 

3.88-6.47 mmol/L

Fraction of total cholesterol: 0.65-0.75

Cholinesterase (pseudocholinesterase)

Erythrocytes

Plasma

0.65-1.3 pH units

0.5-1.3 pH units

8-18 U/L at 37EC

1

1

0.65-1.3 pH unitsŠ

0.5-1.3 pH units

8-18 U/L at 37EC

Citrate

Serum or Plasma

1.7-3.0 mg/dL

52.05

88-156 mmol/L

Copper

Serum or Plasma

Male

Female

 

70-140 Fg/dL

80-155 Fg/dL

 

0.1574

 

11-22 mmol/L

13-24 mmol/L

Cortisol

Plasma

8am-10am

4pm-6pm

 

5-23 Fg/dL

3-13 Fg/dL

 

27.59

 

138-635 nmol/L

83-359 nmol/L

Creatine

Serum or Plasma

Male

Female

 

0.1-0.4 mg/dL

0.2-0.7 mg/dL

 

76.25

 

8-31 mmol/L

15-53 mmol/L

Creatine kinase(CK)

Serum

Male

Female

 

55-170 U/L at 37EC

30-135 U/L at 37EC

 

1

1

 

55-170 U/L at 37EC

30-135 U/L at 37EC

Creatinine

Serum or Plasma

0.6-1.2 mg/dL (adult)

0.3-0.6 mg/dL (children <2y)

88.40

53-106 mmol/L

27-53 mmol/L

Creatinine clearence (endogenous)

Serum or Plasma and Urine

Male

Female

 

 

107-139 mL/min

87-107 mL/min

 

 

0.01667

 

 

1.78-2.32 mL/s

1.45-1.78 mL/s

Cryoglobulins

Serum

Negative

---

Negative

Electrophoresis, protein

Albumin

Alpha-1

Alpha-2

Beta

Gamma

Albumin

Alpha-1

Alpha-2

Beta

Gamma

Serum

 

 

 

 

Serum

 

 

52-65% of total protien

2.5-5.0% of total protien

7.0-13.0% of total protien

8.0-14.0% of total protien

12.0-22.0% of total protien

Concentration

3.2-5.6 g/dL

0.1-0.4 g/dL

0.4-1.2 g/dL

0.5-1.1 g/dL

0.5-1.6 g/dL

 

0.01

0.01

0.01

0.01

0.01

10

 

Fraction of total protien: 0.52-0.65

0.025-0.05

0.07-0.13

0.08-0.14

0.12-0.22

32-56 gL

1-4 gL

4-12 gL

5-11 gL

5-16 gL