[Frontiers in Bioscience, Landmark, 25, 1974-1984, June 1, 2020]

Curcumin inhibits high glucose oxidative stress and apoptosis in pancreatic beta cells via CHOP/PCG-1α and pERK1/2

Kaijian Hou1#, Yongru Chen2#,Dan Zhu1,Genben Chen 3, Fengwu Chen1, Ningning Xu6, Khan Barkat4, Jiehong Zheng1, Xi Xie5, Rongping Chen6

1Department of Endocrine, Longhu People's Hospital, Shantou, Guangdong, China, 2Department of Emergency Intensive Care Unit(EICU), First Affiliated Hospital of Medical College of Shantou University, Dongxia S Rd, Jinping Qu, Shantou Shi, Guangdong Sheng, China, 3Department of Endocrine, Chao'an District People's Hospital, Chaozhou, Guangdong, China, 4Department of Pharmaceutics, Gomal University, Dera Ismail Khan, Khyber Pakhtunkhwa, Pakistan, 5Department of Endocrinology, Shantou Traditional Chinese Medicine Hospital, 6Department of Endocrinology, Zhujiang Hospital Affiliated to Southern Medical University, Guangzhou, Guangdong, China


1. Abstract
2. Introduction
3. Materials and methods
    3.1. Reagents
    3.2. Cell culture
    3.3. Transient transfection
    3.4. Quantitative polymerase chain reaction (qPCR)
    3.5. Western blotting
    3.6. Assessment of oxidative stress
    3.7. Assessment of apoptosis
    3.8. Statistical analysis
4. Results and discussion
    4.1. Curcumin suppresses HG induced oxidative stress and apoptosis in pancreatic beta cells
    4.2. Curcumin reduces oxidative stress and apoptosis by regulation of CHOP and PGC-1α
    4.3. Curcumin regulates the phosphorylation of ERK 1/2 and reduces oxidative stress and apoptosis
5. Acknowledgments
6. References


Loss of pancreatic beta-cells by apoptosis appears to play an important role in the development of insulin deficiency and the onset and/or progression of the diabetes mellitus. To this end, we report that curcumin prevents high glucose (HG) induced oxidative stress and apoptosis in mouse pancreatic beta cells. Moreover, curcumin prevents HG induced increase in expression of CHOP, decrease in PCG-1α and phosphorylation of ERK1/2 (pERK1/2) without any effect on the phosphorylation levels of p38 and JNK. Moreover, similar to curcumin, blockade of pERK1/2 reduced the HG induced apoptosis in pancreatic beta cells. Overexpression of CHOP or siRNA knockdown of PCG-1α counteracted the effect of curcumin on HG induced apoptosis and oxidative stress. These results suggest that curcumin acts through CHOP/PCG-1α and ERK1/2 signaling to block the HG induced oxidative stress and apoptosis.


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Abbreviations: Real-time quantitative polymerase chain reaction: RT-qPCR; reactive oxygen species: ROS; malondialdehyde: MDA; superoxide dismutase: SOD; diabetes mellitus: DM; high glucose: HG; type 2 diabetes mellitus: T2DM; Dulbecco's Modified Eagle Medium F-12: DMEM/F12; fetal bovine serum: FBS; bovine serum albumin: BSA

Key Words Diabetes mellitus; Pancreatic beta cells, Curcumin, Apoptosis; Oxidative stress, CHOP; PGC-1αlpha, MAPK pathway

Send correspondence to: Rongping Chen, Department of Endocrinology, Zhujiang Hospital Affiliated to Southern Medical University, Shantou, Guangdong, PR China, Tel: 13632101107, Fax: 020-61643195, E-mail: 62782333@163.com