[Frontiers in Bioscience S5, 631-637, January 1, 2013]

Designing a peptide-based vaccine against Porphyromonas gingivalis

Alberta Lucchese1, Agostino Guida1, Giovanni Capone2, Massimo Petruzzi3, Dorina Lauritano4, Rosario Serpico1

1Department of Odontostomatological, Orthodontics and Surgical Disciplines, Second University of Naples SUN, Naples, Italy, 2Department of Biosciences, Biotechnologies and Pharmacological Sciences, University of Bari, Bari, Italy, 3Dental Clinic, University of Bari, Bari, Italy, 4Neuroscience Department, Milano-Bicocca University, Monza, Monza e Brianza, Italy


1. Abstract
2. Introduction
3. Methods
4. Results
4.1. Peptide-peptide profiling of Porphyromonas gingivalis fimA type I protein versus the human proteome
4.2. Peptide sequences unique to Porphyromonas gingivalis fimA type I protein: immunogenicity analysis
5. Discussion
6. References


Using proteome databases and exploiting the concept that a rare sequence is a potential epitope, epitopic sequences derived from Porphyromonas gingivalis fimA type I protein were examined for pentapeptide sequence similarity score to the human proteome. We obtained data showing that most of the linear bacterial determinants are (or are formed by) peptide fragment(s) absent (or rarely found) in the human proteins. These results seem to confirm the hypothesis that low-sequence similarity may contribute to shape the epitope repertoire and provides a potential tool for designing new immunotherapeutic approaches to apply in Porphyromonas gingivalis infected periodontitis.