[Frontiers in Bioscience E5, 517-524, January 1, 2013]

Real-time imaging of mouse lenticulostriate artery following brain ischemia

Falei Yuan1, Yongting Wang1, Yongjing Guan1, 2, Yuqi Ren3, Haiyan Lu1, Tiqiao Xiao3, Honglan Xie3, Peter S. Vosler4, Jun Chen4, Guo-Yuan Yang1, 2

1Neuroscience and Neuroengineering Research Center, Med-X Research Institute, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China, 2Ruijin Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200030, China, 3Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, China, 4Department of Neurology and Pharmacology, University of Pittsburgh, Pittsburgh, PA 15213, USA


1. Abstract
2. Introduction
3. Materials and methods
3.1. Middle cerebral artery occlusion
3.2. SR parameters
3.3. SR imaging
3.3.1. Cerebral microangiography in situ
3.3.2. Cerebral microangiography in vivo
3.4. CT imaging
3.5. Image processing
4. Results
4.1. Characteristics of LSAs
4.2. CT reconstruction
4.3. Ischemia/reperfusion induced hemorrhage due to LSA rupture
5. Discussion
6. Acknowledgements
7. References


Detection of microvascular changes in experimental stroke models is limited by current technologies. Using state-of-the-art synchrotron radiation (SR), we explored the feasibility of detecting the normal morphological variations of lenticulostriate arteries (LSAs) and the changes to LSAs following middle cerebral artery occlusion (MCAO). Cerebral microvessels of ICR mice were imaged with synchrotron radiation microangiography using nonionic iodine and barium sulfate as contrast agents. Using SR we reproducibly observed the detailed cerebral microvasculature of LSAs arising from the origin of middle cerebral artery (MCA) with a resolution of approximately 5 micrometers, at least a 20-fold greater resolution compared to CT or MRI imaging. Notably, SR microangiography was able to reveal ischemia/reperfusion induced leakage in the lenticulostriate artery territory. To our knowledge this is the first time that the three-dimensional morphology of LSAs and real time visualization of LSA hemorrhage have been characterized in live mice. This work demonstrates that SR microangiography can provide a unique tool for furthering experimental stroke research to examine the efficacy of neuroprotective therapies on parameters such as angiogenesis and vascular integrity.