[Frontiers in Bioscience E5, 266-272, January 1, 2013]

Experimental models of lung transplantation

Alejandro Cano Bribriesco1, Wenjun Li1, Ruben Guadalupe Nava1, Jessica Helen Spahn1, Daniel Kreisel1,2

1Department of Surgery Washington University in St. Louis 660 South Euclid Avenue, St. Louis, MO 63110-1013, 2Department of Pathology and Immunology, Washington University in St. Louis 660 South Euclid Avenue, St. Louis, MO 63110-1013

TABLE OF CONTENTS

1. Abstract
2. Introduction
3. Canine lung transplantation models as a platform for development of surgical techniques and immunosuppression
4. Orthotopic lung transplantation in rats
5. Orthotopic porcine lung transplantation models
6. Tracheal transplantation models
7. Orthotopic lung transplantation in the mouse
8. Conclusions
9. References

1. ABSTRACT

Lung transplantation is a life saving treatment for end stage pulmonary diseases. The development and refinement of this therapy required the utilization of various animal models, without which this procedure would not have become a clinical reality. Canine models were critical in the initial breakthroughs in surgical technique and immunosuppressive regimens, which directly led to the first successful human lung transplantation. Orthotopic lung transplant models in the rat provided a platform for more detailed investigation of immune responses to pulmonary grafts. Investigation of chronic rejection of lungs has significantly been advanced through the use of mouse tracheal transplant experiments. And finally, the advent of orthotopic, vascularized lung transplantation in the mouse opened the door to the use of genetic and molecular tools that are necessary for the rigorous mechanistic study of alloimmune and non-alloimmune factors contributing to lung graft failure. Taken together, animal models will continue to be a cornerstone in the advancement of clinical success in lung transplantation.