[Frontiers in Bioscience E3, 830-833, June 1, 2011]

Isolation and identification of novel geosmin-degrading bacteria

Qiang Xue1, Gang Chen1, Kazuya Shimizu2, Meena Kishore Sakharkar1, Motoo Utsumi1, Honghan Chen3, Miao Li 3, Zhenya Zhang1, Norio Sugiura1

1Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572 Japan, 2Faculty of Life Sciences, Toyo University, Gunma 374-0193, Japan, 3School of Water Resources and Environment, China University of Geosciences (Beijing), Beijing 100083, China


1. Abstract
2. Introduction
3. Materials and methods
3.1. Geosmin standard
3.2. Enrichment and isolation
3.3. Degradation potential check
3.4. Kinetic study
3.5. Geosmin concentration estimation by GC/MS
3.6. Phylogenetic analysis
4. Results and discussion
4.1. Test for degradation potential
4.2. Kinetic analyses
4.3. Phylogenetic analysis
5. Conclusion
6. Acknowledgements
7. References


Three novel geosmin-degrading bacteria were isolated from the sediments of Lake Kasumigaura, Japan. All strains were identified as Acinetobacter spp. by 16S rRNA gene sequence analysis and can biodegrade geosmin at an initial geosmin concentration of 2 mg/L after 2 days. Furthermore, at an initial geosmin concentration of 40 g/L, geosmin removal was more than 68% by GSM-2 strain, and the degradation mechanism followed a pseudo-first-order mode. A rate constant of 0.026 reveals rapid geosmin degradation. This is the first report on geosmin degradation by by Acinetobacter spp.