[Frontiers in Bioscience 14, 210-224, January 1, 2009]

Stromal cells

Muriel Vayssade, Marie-Danielle Nagel

Domaine Biomateriaux-Biocompatibilite, CNRS UMR 6600, Universite de Technologie de Compiegne, BP 20529 60205 Compiegne Cedex, France


1. Abstract
2. Introduction
3. Interactions between stromal cells and their environment, and cell-cell interactions in the real in vivo environment
3.1. Stromal cells
3.1.1. Denominations used to refer to stromal cells
3.1.2. Sources of stromal cells
3.1.3. Characterization of stromal cells
3.2. Differentiation pathways
3.3. In vivo functions of stromal cells
3.3.1. HSC differentiation
3.3.2. Host immune response
3.3.3. Homing mechanisms
4. Interactions between stromal cells and materials : biohybrid systems
4.1. Materials
4.1.1. Natural materials Titanium Ceramics Hydroxyapatite Tricalcium phosphate Animal bone or skeleton Biomolecules Hyaluronic acid Matrigel Collagen Silk Alginate Chitosan
4.1.2. Synthetic materials
4.2. Bioreactors used to culture stromal cells
5. Conclusions
6. References


Stromal cells, or mesenchymal stem cells, are adherent clonogenic cells that can form colonies. They are mainly isolated from bone marrow but can also be found in umbilical cord blood, adipose tissues and amniotic fluids. These stem cells are easy to culture in vitro, and can differentiate into osteoblasts, chondrocytes, or adipocytes when stimulated appropriately. When seeded on a natural (titanium, ceramics, collagen fibers, silk, etc.) or synthetic (PLLA, PLGA, etc.) biomaterial scaffold, they adhere and differentiate to form a new tissue. Many studies have also explored stromal cell differentiation in bioreactors to form a 3-dimensional culture. This review focuses on the biomaterials used for tissue engineering with stromal cells.