[Frontiers in Bioscience 3, a38-46, June 9, 1998]
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CHARACTERIZATION OF KINETICS OF ANTI-TRICHINELLA SPIRALIS NEWBORN LARVAE IMMUNITY IN RATS

Ching-Hua Wang

Biology Department, California State University, 5500 University Parkway, San Bernardino, CA 92407, USA

Received 5/11/98 Accepted 5/24/98

TABLE OF CONTENTS

1. Abstract
2. Introduction
3. Materials and methods
3.1. Experimental animals and infection
3.2. Examination of muscle larvae burden
3.3. Recovery of newborn larvae from peritoneal fluid and portal vein blood
3.4. Determination of cell adherence to newborn larvae and viability of newborn larvae
3.5. Quantitation of immune protection against newborn larvae challenge
3.6. Kinetics of cuticular cell adherence estimated in vitro and in vivo
3.7. Effect of immunization dose on anti-newborn larvae immunity
3.8. Statistical analysis
4. Results
4.1. Kinetics of host anti-newborn larvae immunity determined by muscle larvae burden
4.2. Kinetics of newborn larvae recovery from the peritoneal cavity of immune rats
4.3. Development of cell adherence to newborn larvae in the peritoneal cavity of immune rats
4.4. Development of cuticular cell adherence after in vitro incubation with blood from immunized rats
4.5. The influence of dose of muscle larvae on anti-newborn larvae immunity
4.6. The effects of different doses of muscle larvae infection on anti-newborn larvae immunity detected on day 9 and day 16 of T. spiralis infection
4.7. The effect of different doses of newborn larvae challenge on anti-newborn larvae immunity detected in the peritoneal cavity
5. Discussion
6. Acknowledgment
7. References
8. Entire manuscript

Key words: Mucosal immunity, Trichinella spiralis, Newborn Larvae, Peritoneal Cavity, Cell Adherence, Cytotoxicity, Rat

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