[Frontiers in Bioscience, 2, e1-11, April 1, 1997]
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CAVEAT LECTOR




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TRANSGENIC APPROACHES FOR THE REDUCTION IN EXPRESSION OF GALalpha(1,3)GAL FOR XENOTRANSPLANTATION

Mauro S. Sandrin, Narin Osman, Ian F.C. McKenzie

Molecular Immunogenetics Laboratory, Austin Research Institute, Austin & Repatriation Medical Center, Heidelberg Vic. 3084, Australia

Received 12/31/96; Accepted 3/12/97; On-line 4/1/97

TABLE OF CONTENTS

1. Abstract
2. Introduction
3. Hyperacute rejection
4. The major xenoepitope: Galalpha(1,3)Gal
4.1 Carbohydrate inhibition
4.2 Absorption studies
4.3 Transfection studies
4.4 In vivo studies
4.5 Are all the human anti-pig antibodies directed to Galalpha(1,3)Gal?
4.6 Cellular and molecular distribution of Galalpha(1,3)Gal
5. alpha1,3Galactosyltransferase
6. Elimination of Galalpha(1,3)Gal by gene inactivation strategies
7. Elimination of Galalpha(1,3)Gal by transgenic strategies
7.1 The alpha1,2Fucosyltransferase enzyme
7.1.1. Expression in COS cells
7.1.2. Expression in porcine cells
7.1.3. Expression in transgenic mice
7.1.4 . Expression in transgenic pigs
7.1.5 Molecular mechanisms for suppression of Galalpha(1,3)Gal production
7.1.6 Secretor type alpha1,2fucosyltransferase
7.2. alphaGalactosidase
7.2.1 Expression of alphagalactosidase cDNA in COS cells
7.2.2 Expression of alphagalactosidase cDNA in a pig cell line
7.2.3 In vivo expression of alphagalactosidase
7.3 Combination of alpha1,2fucosyltransferase and alphagalactosidase
8. Conclusions
9. References
10. Entire manuscript

Key Words: xenotransplantation, Galalpha(1,3)Gal, transgenesis, glycosyltransferase.