[Frontiers in Bioscience 2, c15-29, September 1, 1997]

	[Frontiers in Bioscience 2, c15-29, September 1, 1997 Reprints PubMed CAVEAT LECTOR
	IN SITU PCR. OVERVIEW OF PROCEDURES AND APPLICATIONS Carlos A. Muro-Cacho, M.D., Ph.D. Department of Pathology, H. Lee Moffitt Cancer Center and Research Institute and University of South Florida College of Medicine, Tampa, Florida, USA Received 1/10/97 Accepted 7/15/97 TABLE OF CONTENTS 1. Abstract 2. Introduction 3. General considerations 3.1. Equipment 3.2 Technical aspects 3.3 Types of samples 3.4 Fixation 3.5 Proteolytic digestion 3.6 Additional pretreatments 4. Polymerase Chain Reaction (PCR) 4.1 Polymerases 4.2 Deoxynucleotide triphosphates (dNTPs) 4.3 Buffers 4.4 Primers 4.5 Cycling profile 5. In situ PCR Protocols 5.1 In situ RT-PCR 5.2 Direct In situ PCR 5.3 Indirect In situ PCR 6. Controls 6.1 Causes of false negativity 6.2 Causes of false positivity 6.3 DNA repair 6.4 Mispriming 6.5 Endogenous priming 6.6 Diffusion of PCR products 7. Applications 8. Acknowledgment 9. References 10. Appendices Appendix 1 - General information Appendix 2 - Sample preparation Appendix 3 - Proteolytic digestion Appendix 4 - Additional pretreatments Appendix 5 - RT-PCR Appendix 6 - Polymerase Chain Reaction Appendix 7 - Direct In situ PCR Appendix 8 - Indirect In situ PCR Appendix 9 - Detection methods 11. Entire manuscript Keywords: PCR, In situ PCR Search OMIM (Online Mendelian Inheritance in Man) to find related articles This form requires a WWW Client such as Netscape, XMosaic, Lynx that supports fill-in forms. Enter one or more search keywords: To Search all fields, leave the following boxes unchecked. To narrow your search to certain specific fields, check the boxes next to those fields' names. SeeQuery Help for other ways to search. Title: OMIM Number: Allelic Variants: Text: References: Clinical Synopsis: Gene Map Disorder: Contributors: See only records which have been changed in the past Display at most

[Frontiers in Bioscience 2, c15-29, September 1, 1997
Reprints
PubMed
CAVEAT LECTOR

IN SITU PCR. OVERVIEW OF PROCEDURES AND APPLICATIONS

Carlos A. Muro-Cacho, M.D., Ph.D.

Department of Pathology, H. Lee Moffitt Cancer Center and Research Institute and University of South Florida College of Medicine, Tampa, Florida, USA

Received 1/10/97 Accepted 7/15/97

TABLE OF CONTENTS

1. Abstract
2. Introduction
3. General considerations: 3.1. Equipment; 3.2 Technical aspects; 3.3 Types of samples; 3.4 Fixation; 3.5 Proteolytic digestion; 3.6 Additional pretreatments
4. Polymerase Chain Reaction (PCR): 4.1 Polymerases; 4.2 Deoxynucleotide triphosphates (dNTPs); 4.3 Buffers; 4.4 Primers; 4.5 Cycling profile
5. In situ PCR Protocols: 5.1 In situ RT-PCR; 5.2 Direct In situ PCR; 5.3 Indirect In situ PCR
6. Controls: 6.1 Causes of false negativity; 6.2 Causes of false positivity; 6.3 DNA repair; 6.4 Mispriming; 6.5 Endogenous priming; 6.6 Diffusion of PCR products
7. Applications
8. Acknowledgment
9. References
10. Appendices: Appendix 1 - General information; Appendix 2 - Sample preparation; Appendix 3 - Proteolytic digestion; Appendix 4 - Additional pretreatments; Appendix 5 - RT-PCR; Appendix 6 - Polymerase Chain Reaction; Appendix 7 - Direct In situ PCR; Appendix 8 - Indirect In situ PCR; Appendix 9 - Detection methods
11. Entire manuscript

Keywords: PCR, In situ PCR

Search OMIM (Online Mendelian Inheritance in Man) to find related articles
This form requires a WWW Client such as Netscape, XMosaic, Lynx that supports fill-in forms.