[Frontiers in Bioscience 1, e34-41, July 1, 1996]
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CAVEAT LECTOR



PIG alpha1,3GALACTOSYLTRANSFERASE: A MAJOR TARGET FOR GENETIC MANIPULATION IN XENOTRANSPLANTATION

Karen Strahan, Andrew Preece and Kenth Gustafsson

Division of Cell & Molecular Biology, Institute of Child Health, University of London, 30 Guilford St., London WC1N 1EH, UK

Received 06/21/96; Accepted 07/02/96; On-line 07/08/96

TABLE OF CONTENTS

1. Abstract
2. Introduction
3. Gala1,3Gal: the major target for human anti-pig NAb
4. Evolution and possible function of the Gala1,3Gal epitope
4.1. Evolution of the gene encoding the a1,3GT
4.2. The possible functional significance of the Gala1,3Gal epitope
5. Down-regulation or obliteration of the Gala1,3Gal epitope
5.1. Knock-out of the gene encoding a1,3GT
5.2. a1,2FT competition with the substrate for a1,3GT
5.3. Oligonucleotide antisense inhibition of a1,3GT expression
5.4. Ribozymes as tools for down-regulation of the a1,3GT expression
6. Concluding remarks
7. Acknowledgments
8. References
9. Entire manuscript

Key Words: Xenotransplantation, Endothelial cell, a1,3galactosyltransferase, Natural Antibodies, Gene knock-out, Antisense, Ribozyme