[Frontiers in Bioscience 1, d146-160, August 1, 1996]
Reprints
PubMed
CAVEAT LECTOR



INTERACTION OF HUMAN SPERMATOZOA WITH THE ZONA PELLUCIDA OF OOCYTE: DEVELOPMENT OF THE ACROSOME REACTION

Patricio Morales1 and Miguel Llanos2

1 P. Catholic University of Chile, Faculty of Biological Sciences. P. O. Box 114-D. Santiago, Chile.

2INTA, University of Chile, P. O. Box 138-11. Santiago, Chile.

10. CONCLUDING REMARKS

There are still many unanswered questions regarding mammalian sperm interaction with the oocyte. The requirement for sperm capacitation as well as the acrosomal status of the spermatozoa traveling through the cumulus oophorus needs to be studied further. Although efforts have been made to establish the characteristics of the proteins present in the sperm surface which act as the receptor for ZP3 glycoprotein, the exact function for these proteins in the mechanism associated to the ZP3-induced AR has not yet been elucidated. Thus, future studies should focus on determining the exact nature of these receptors, their structure, and function. The presence of neuronal receptors (such as GABA-like and glycine receptor) in mammalian spermatozoa with a role in the AR, either induced by progesterone or solubilized ZP, has been recently suggested. In addition, the AR, a major regulatory event for mammalian spermatozoa-oocyte interaction leading to fertilization, requires further studies to integrate the information regarding the role of the different signaling pathways. These studies may provide the precise sequential role of the intracellular messengers produced after the spermatozoa-ZP interaction has taken place. If previously reacted spermatozoa can initiate binding to the ZP, one major question is whether they are able to penetrate the ZP and fertilize the oocyte. The nature of the zona (ZP2) receptor in the acrosomal-reacted spermatozoa and the acrosomal proteases in the development of the AR and penetration through the ZP need to be clarified. Investigation of these and other related questions should unravel the molecular processes involved in the acrosome reaction.

[Table of Contents ] [Next Section] [Previous Section]