Ned J.C.Lamb, Cecile Gauthier-Rouviere and Anne Fernandez.

Cell Biology Unit, C R B M , CNRS-INSERM, 1919, Route de Mende, F-34033, Montpellier Cedex. France.

Received 15/12/95; Accepted 30/01/96; On-line 03/01/96

Key words: mammalian cells, growth control, micro-needle microinjection

Abbreviations: SRF: Serum Response Factor; SRE: Serum Response Element; TRE: TPA Response Element; A-kinase: cAMP-dependent protein kinase; C-kinase: Calcium/phospholipids-dependent kinase; CKII: Casein kinase II; PKI: Protein kinase Inhibitor of A-kinase; C-PKI: inhibitor peptide for c-kinase

TABLE OF CONTENTS

1. Abstract

2.1 Microinjection of oligonucleotide for in vivo competition in the study of DNA binding transcription factors.

2.2 Microinjection of antibody for in vivo study of transcription factors activity.

2.3 Dominant negative mutant of transcription factors based upon their DNA binding domain.

3.1 Microinjection of purified kinases and phosphatases to investigate the regulatory mechanisms involved in mitogenic activation.

3.2 Microinjection of proteins and peptides inhibitory to kinases and phosphatases.

4.1 Microinjection of epitope-tagged or normal expression plasmids.

4.1.1 Microinjection of plasmid to overexpress proteins.

4.1.2 Microinjection of plasmid to produce antisense RNA.

5. Concluding Remarks.

6. Acknowledgments

7. References

8. Entire manuscript